Objective:To study the active fractions in liver protection from Erzhi Pill(AFEP) on acute hepatic injury induced by carbon tetrachloride(CCl4)in mice. Method: Sixty Kunming mice were randomly divided into six groups:the normal group

the model group

bifendate group (150 mg·kg-1)

high AFEP group (19.8 g·kg-1)

middle AFEP group (13.2 g·kg-1)and low AFEP group (6.6 g·kg-1).The treatment groups were orally administered once per day for 7 d separately

whereas the normal and model groups were orally administered with saline. Except normal rats

all the other rats were injected intraperitoneally CCl4 20 mL·kg-1 once. The rats were sacrificed 16 h after CCl4 administration. Serum and liver samples were collected for analysis. The acute hepatic injury model was prepared by CCl4 injected intraperitoneally. Then

the therapeutic effects of AFEP on the model were evaluated by the activity determination of serum alanine aminotransferase(ALT) and aspirate aminotransferase (AST)

superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in liver

and the hepatic pathohistological changes following the treatment. Result: The activities of ALT and AST and the MDA content in liver was significantly increased and the activity of SOD was largely inhibited in the animals of modeling group. Following the treatment with AFEP

ALT and AST activities and MDA content were significantly reduced and SOD activity was obviously increased in the mice of treatment group.Furthermore

AFEP could ameliorate the hepatic pathological changes. Conclusion: AFEP have protective effects on acute hepatic injury induced by CCl4 in mice

and are the effect of the liver protecting active sites.