Objective: A rapid and specific high performance liquid chromatography-mass spectrometric method was developed for determination of 17β-tenacigenin B and tenacigenoside A in Xiaoaiping injection. Method: The analytical column was Agilent Zorbax SB-C18 (3.0 mm×100 mm

3.5 μm) and the security guard column was Agilent Zorbax SB-C18 (2.1 mm×12.5 mm

5 μm). The mobile phase was methanol-water (90∶10) at a flow rate of 0.25 mL·min-1. Methyltestosterone was used as internal standard; electrospray ionization model (ESI) and SIM model were used for quantification. Result: The linear ranges of two compounds were as follows: 0.625-20 mg·L-1 (r=0.998 3) for 17β-tenacigenin B

0.375-12 mg·L-1 (r=0.999 4) for tenacigenoside A. The average recoveries were 100.8% (RSD 3.95%)

101.6% (RSD 2.71%)

respectively. Conclusion: This established method was sensitive

fast

accurate and available for the quality control of Xiaoaiping Injection.