Objective: To study the effect of total Saponin of Radix Clematidis (TSRC) on HL60 leukemia cell in vitro. Method: In vitro

HL60 cells were incubated with 25-400 mg·L-1TSRC

the proliferation inhibition rate of HL60 was tested by WST-1.Cell morphology

transmission electron microscope(TEM) and Annexin-V/PI were used to investigate the apoptosis of HL60 cells. The differentiations of HL60 was analyzed by morphology of Giemsa stain

nitroblue tetrazdium(NBT) reduced method. The expressions of CD11b and CD33 induced by these drugs were measured by flow cytometry(FCM) at different time. Result: As compared with blank control group

Different concentrations from 50-400 mg·L-1 TSRC could obviously inhibit the growth of HL60 cells with time and dose dependence; While no statistical significance in the differentiation phenomenon was observed. Conclusion: In vitro

HL60 cell’s proliferation inhibition and apoptosis could obviously induced by TSRC. But HL60 cells could not be induced differentiation by TSRC.