穿山龙总皂苷对白介素-1诱导大鼠成纤维样滑膜细胞PI3K/AKT的影响

于栋华; 刘磊; 卢芳; 杨超; 薛欢欢; 刘树民

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穿山龙总皂苷对白介素-1诱导大鼠成纤维样滑膜细胞PI3K/AKT的影响

更新时间：2024-01-04

- 穿山龙总皂苷对白介素-1诱导大鼠成纤维样滑膜细胞PI3K/AKT的影响
- Effect of Total Saponin of Dioscoreae Nipponicae Rhizoma on PI3K/AKT Signal Pathway by in rIL-1 Induced Fibroblast-Like Synoviocytes
- 中国实验方剂学杂志 2012年18卷第23期页码：199-202
- 作者机构：
- 作者简介：
- 基金信息：
  
  黑龙江省青年科学基金项目(QC2009C46,QC2011C109)
- DOI：
  中图分类号：
- 纸质出版日期：2012
- 稿件说明：
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于栋华, 刘磊, 卢芳, 等. 穿山龙总皂苷对白介素-1诱导大鼠成纤维样滑膜细胞PI3K/AKT的影响[J]. 中国实验方剂学杂志, 2012,18(23):199-202.

YU Dong-hua, LIU Lei, LU Fang, et al. Effect of Total Saponin of Dioscoreae Nipponicae Rhizoma on PI3K/AKT Signal Pathway by in rIL-1 Induced Fibroblast-Like Synoviocytes[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(23): 199-202.
于栋华, 刘磊, 卢芳, 等. 穿山龙总皂苷对白介素-1诱导大鼠成纤维样滑膜细胞PI3K/AKT的影响[J]. 中国实验方剂学杂志, 2012,18(23):199-202. DOI：

YU Dong-hua, LIU Lei, LU Fang, et al. Effect of Total Saponin of Dioscoreae Nipponicae Rhizoma on PI3K/AKT Signal Pathway by in rIL-1 Induced Fibroblast-Like Synoviocytes[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(23): 199-202. DOI：

摘要

目的: 观察穿山龙总皂苷对重组白介素-1β(rIL-1β)诱导的大鼠成纤维样滑膜细胞(FLS)的影响

探讨其对PI3K/AKT通路的作用。方法: 原代培养滑膜细胞;模型组给予10 μg·L-1 rIL-1β

给药组给予10 μg·L-1 rIL-1β和100 μg·L-1穿山龙总皂苷共同孵育

孵育72 h后用Western blot法测定穿山龙总皂苷对rIL-1β诱导大鼠FLS中磷脂酰肌醇-3激酶(p-PI3K)、蛋白激酶B(p-AKT)表达的影响。结果: 与空白组比较

模型组p-PI3K

p-AKT水平明显升高(分别为162.66±2.34

157.03±1.84)(P＜0.05)

穿山龙总皂苷能显著降低rIL-1β诱导下大鼠FLS中p-PI3K

p-AKT水平(分别为67.84±1.24

71.66±1.20)(P＜0.01)。结论: 穿山龙总皂苷可抑制PI3K/AKT信号转导通路;穿山龙总皂苷具有治疗痛风性关节炎(Gouty Arthritis

GA)的潜在价值。

Abstract

Objective: To explore the effects of total saponin of Dioscoreae Nipponicae Rhizoma(RDN) on in vitro apoptosis of rIL-1β induced fibroblast-like synoviocytes(FLS)

and further to investigate the PI3K/AKT signal mechanism. Method: FLS were primarily cultured. Model group: 10 μg·L-1 rIL-1β

RDN group: 10 μg·L-1 rIL-1β and 100 μg·L-1 RDN. Western blot method was applied to examine the expression of p-PI3K protein and p-AKT protein degradation in rIL-1β induced FLS stimulated with total saponin of RDN. Result: Compared to control group

expression of p-PI3K and p-AKT protein increased in model group

respectively 162.66±2.34 and 157.03±1.84(P＜0.05)

total saponin of RDN inhibited rIL-1β induced phosphorylation of AKT and PI3K in FLS

respectively 67.84±1.24 and 71.66±1.20 (P＜0.01). Conclusion: Total saponin of RDN can inhibit FLS PI3K/AKT signal pathway. Total saponin of RDN may provide a new therapeutic approach in treatment of gouty arthritis.

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