Objective:To found HPLC method for the determination of nomadic gallic acid in diyu tannin extract

then offer some references for the extraction process and quality standard study of diyu tannin. Method: The chromatographic condition was investigated. The methods of preparing sample solution

specialization

linearity and linear range

precision

stability

recovery and the content of samples was determined. Result: The content of nomadic gallic acid was determined at 272 nm detection wavelength with methyl alcohol-0.05% phosphoric acid aqueous solution(5∶95)as mobile phase

the flow rate was 1.0 mL·min-1

and the column temperature was at 30 ℃. The methods of preparing sample solution

specialization

linearity and linear range

precision

stability

recovery of this method all met the relative requirements. The content of gallic acid in the preparing sample ranged from 3.21% to 4.05%

the average was 3.71%. Conclusion: The results indicated that above-mentioned method was not only scientific

reasonable

feasible

but simple

rapid and accurate as well; what follows next

it can provide the basis for the relative study of tannins.