Objective: To investigate human immunodeficiency virus(HIV/AIDS) in urine of patients with metabolic changes and to explore future application value of metabonomics technique in research of preventing and delaying the acquired immunodeficiency syndrome. Method: Using liquid chromatography coupled to mass spectrometry (LC-MS)

urine metabolic components in 55 HIV/AIDS patients and 20 healthy human beings were studied by metabonomics method

and combined with principal component analysis (PCA) and t test analysis technology

the specific metabolic substances (or group spectrum)were analyzed. Result: The urine metabolic profiles in the two groups showed differences with 10 potential biological markers. Conclusion: From the metabonomics method

HIV/AIDS patients and healthy people can be well differentiated. Comprehensive metabolic markers indicate HIV/AIDS metabolic characteristics. Metabonomics approach in the study of prevention and delay of HIV virus infection will have good prospects.