Objective:To investigate effects of Buyang Huanwu decoction containing serum on the morphology and viability of PC12 cells during oxygen-glucose deprivation. Method:Buyang Huanwu decoction containing serum was obtained by the seropharmacological method. PC12 cells were treated with different concentrations (5%

10%

20%) of the serum during oxygen-glucose deprivation. The cell morphology was observed with inverted microscopy

and the cell viability was examined by methyl thiazolyl tetrazolium assay. Result:The cell morphology was changed and the cell viability was decreased during oxygen-glucose deprivation

and the injured degree of cell morphology was relieved and the cell viability was increased in PC12 cells which treated with concentrations of 5%

10% Buyang Huanwu decoction medicated serum for 12

18

24 h(P<0.01). However

20% Buyang Huanwu decoction containing serum had the bidirectional regulation effects on the morphology and viability of PC12 cells during oxygen-glucose deprivation. Conclusion:The certain concentration of Buyang Huanwu decoction congtaining serum has protective effects on the morphology and viability of PC12 cells during oxygen-glucose deprivation.