Objective: To investigate the effect of Gansui Banxia decoction with different proportion of KANSUI RADIX and Gansui and GLYCYRRHIZAE RADIX ET RHIZOMA in malignant ascites rats

and then find out the compatibility proportion when it works. Method: We set up seven matched groups according to the principle of uniform design(two factors seven levels) compared with normal

model

positive group. At the end of experiment

we measured urine volume with the cylinder

caculated ascite fluid volume through differential value of the body weight of rats with or without ascites

and determined the content of tumor necrosis factorα(TNF-α) and vascular endothelial growth factor (VEGF) in ascites by radioimmunoassay. The data statistic analysis was processed by analysis of variance (ANOVA) in combination with optimized formula soft. Result: Matched group1 could increase urine output(P<0.05)

and matched group 6

group 7 could decrease the content of TNF-α and VEGF(P<0.05). Conclusion: When the ratio is 15: 1 in compatibility of gansui and gancao

it could increase urine output

and inhibit cytokine in malignant ascites.