Objective: To establish a sensitive ultraperformance liquid chromatography(UPLC) method for determining the plasma concentration of paeonol in liver cancer model rat and to evaluate its pharmacokinetic characteristics. Method: Rats were divided into three groups

a single ig dose of 259.2

194.4

129.6 mg·kg-1 paeonol was given respectively.Paeonol was separated on a C18 column with methanol:water(70: 30) as mobile phase.The plasma concentration of paeonol was determined and its pharmacokinetic parameters were calculated by using WinNonlin 5.2.1. Result: The linear range of the method for paeonol was 0.323-41.4 mg·L-1.The plasma concentration-time course showed two compartment model

with the increased dose

AUC

Cmaxhas a significant increase

while Tmax

T1/2β

CL have no significant change. Conclusion: The UPLC method for determining paeonol concentration in plasma is simple

rapid

sensitive and suitable for pharmacokinetic study. There are some differences between pharmacokinetic parameters in liver cancer model rats with different dose of paeonol.