Objective:To establish the methods for the quantification and quantitation of Kudouzi tablets. Method: Sophora alopecuroides was identified by TLC.The content of matrine was determined by HPLC. HPLC method was performed on a Phenomenex-Pack Gemini-NX 5U C18 110A(4.6 mm×250 mm

5 μm)column with a mobile phase of acetonitrile-0.01 mol·L-1ammonium carbonate water(20∶80).The flow rate was 1 mL·min-1.The wavelength of detector was 220 nm and the column temperature was set at 30 ℃. Result: Characteristics of identification were very obviously.The linear range of matrine was 4.37-26.22 mg·L-1(r=0.999 5) with an average recovery of 102.59%(RSD 0.75%

n=6). Conclusion: The method can be used for the quality and quantity control of Kudouzi tablets.