Objective: To establish the method for simultaneous determination of 6 active components. Method: Simultaneous determination of chlorogenic acid

phellodendrine

magnoflorine

jatrorrhizine

palmatine and berberine in Huangbai was carried out by HPLC. A Diamonsil C18 (4.6 mm×250 mm

5 μm) column was used. The mobile phase was acetonitrile-water (1‰ acetic acid

2 mmol ammonium acetate) solution in gradient elution. The detection wavelength was set at 280 nm

the column temperature was kept at 25℃ and the flow rate was 1 mL· min-1. Result: The linear range of chlorogenic acid

phellodendrine

magnoflorine

jatrorrhizine

palmatine and berberine was 20.00-320.00

18.75-130.00

25.00-200.00

5.00-100.00

20.00-200.00

0.09-1.80 mg· L-1 separately. The average recovery was 98.1%

99.4%

97.5%

97.3%

104.0%

98.5% separately; and RSD was 0.5%

0.6%

0.8%

1.0%

1.4%

0.9% separately. Conclusion: The method is convenient

stable

reliable and suitable for quality control of Huangbai.