两头尖提取物抗猪血清诱导大鼠肝纤维化的作用研究

李孝波; 郝瑞春; 邓晓鹏; 程生辉; 郭继龙; 梁锐; 门九章

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两头尖提取物抗猪血清诱导大鼠肝纤维化的作用研究

更新时间：2024-01-04

- 两头尖提取物抗猪血清诱导大鼠肝纤维化的作用研究
- Protective Effect of Extracts from Rhizoma Anemones Raddeanae on Hepatic Fibrosis Induced by Porcine Serum in Rats
- 中国实验方剂学杂志 2013年19卷第2期页码：201-206
- 作者机构：
- 作者简介：
- 基金信息：
  
  山西省基础研究项目自然科学基金项目(2008011073-2)
- DOI：
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
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李孝波, 郝瑞春, 邓晓鹏, 等. 两头尖提取物抗猪血清诱导大鼠肝纤维化的作用研究[J]. 中国实验方剂学杂志, 2013,19(2):201-206.

LI Xiao-bo, HAO Rui-chun, DENG Xiao-peng, et al. Protective Effect of Extracts from Rhizoma Anemones Raddeanae on Hepatic Fibrosis Induced by Porcine Serum in Rats[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(2): 201-206.
李孝波, 郝瑞春, 邓晓鹏, 等. 两头尖提取物抗猪血清诱导大鼠肝纤维化的作用研究[J]. 中国实验方剂学杂志, 2013,19(2):201-206. DOI：

LI Xiao-bo, HAO Rui-chun, DENG Xiao-peng, et al. Protective Effect of Extracts from Rhizoma Anemones Raddeanae on Hepatic Fibrosis Induced by Porcine Serum in Rats[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(2): 201-206. DOI：

摘要

目的: 观察两头尖提取物对猪血清免疫诱导大鼠肝纤维化模型的干预作用。方法: SD大鼠68只随机分为5组

即空白组、模型组、两头尖提取物组(以下简称提取组)、两头尖原药组(以下简称原药组)、扶正化瘀组(以下简称扶正组)。采用猪血清腹腔注射法制备大鼠肝纤维化模型

0.5 mL/只

每周2次

连续注射15周。第15周模型成功后

开始灌胃给药

扶正组给予扶正化瘀胶囊(0.525 g·kg-1)

原药组给予两头尖煎液(0.7 g·kg-1)

提取物组给予两头尖提取物(0.071 g·kg-1)

模型组与空白组灌服等量生理盐水

日1次

疗程8周。大鼠于第23周末处死取材

分别测定血清丙氨酸氨基转移酶(ALT)

天冬氨酸氨基转移酶(AST)

白蛋白(Alb)

透明质酸(HA)

层黏蛋白(LN)

Ⅲ型前胶原(PCⅢ)

Ⅳ型胶原(CⅣ)含量与肝组织中羟脯氨酸(Hyp)含量。HE染色与Masson法检测肝组织病理;免疫组化法(SABC法)测定肝组织中Ⅰ型胶原(col-Ⅰ)、Ⅲ型胶原(col-Ⅲ)表达。结果: 提取组、原药组血清ALT

AST

Alb

PCⅢ

CIV水平

肝组织Hyp含量分别为(79.77±14.68)

(75.20±11.21)

(168.22±19.46)

(173.72±18.52)U·L-1

(27.40±1.78)

(26.95±2.14)g·L-1

(52.36±5.12)

(43.29±3.56)

(51.63±6.41)

(52.18±4.79)

(14.92±2.26)

(13.93±1.88)μg·L-1

(1.04±0.12)

(1.15±0.06)mg·g-1。与模型组相比

提取组、原药组可显著降低血清ALT

AST

PCⅢ

CIV水平与肝组织Hyp含量(P<0.05)

升高血清Alb水平(P<0.05)。与模型组相比

提取组、原药组炎症活动度、纤维化程度显著减轻。Ⅰ型胶原、Ⅲ型胶原表达面积和强度明显为弱

纤维间隔染色淡

无典型假小叶形成。显色指数结果显示

提取组、扶正组肝组织中col-Ⅰ

col-Ⅲ表达均明显减少。结论: 采用猪血清免疫法可成功制备肝纤维化大鼠模型

两头尖原药及其提取物具有良好的抗肝纤维化作用

且提取物抗肝纤维化作用基本等同于两头尖原药

推测其作用机制为抑制ECM合成并促进ECM降解有关。

Abstract

Objective: To study protective effect of extracts from Rhizoma Anemones Raddeanae(RAR) on hepatic fibrosis induced by pig blood serum in rats. Method: Sixty-eight SD rats were randomly divided into 5 groups

the normal group

the model group

the RAR group

the extraction of RAR group(EXRAR group)

the Fuzheng Huayu group(FZHY group). Rats were intraperitoneally injected with 0.5 mL of porcine serum twice a week to establish immunological liver fibrosis model for 15 successive weeks. The model group was treated with distilled water

the RAR group was treated with RAR decoctum(0.7 g· kg-1)

the EXRAR group was treated with the extraction of RAR(0.071 g· kg-1)

the FZHY group were treated with Fuzheng Huayu gelatin capsule(0.525 g· kg-1).The course of treatment lasted 8 weeks. At the end of 23th weeks. Alanine amino transferase (ALT)

aspartate aminotransferase (AST)

albumin (Alb)

hyaluraic acid (HA)

laminin (LN)

typeⅢ pro collagen (PCⅢ)

collagen Ⅳ(CⅣ) in serum and hydroxyproline (Hyp) content in liver homogenate were assayed. HE stain and Masson stain were used to examine the histopathological change. The expression of collagen Ⅰ(col-Ⅰ)

collagen Ⅲ(col-Ⅲ)were detected by SABC. Result: Compared with the model group

content of ALT

AST

PCⅢ

CIV

Hyp decreased and the content of Alb increased in EXRAR group

RAR group (P<0.05).compared with the model group

level of inflammation activity and fibrosis level was reduced significantly in EXRAR group and RAR group. Immunohistochemistry result indicated that the expression of col-Ⅰ

col-Ⅲ in EXRAR group

RAR group depressed obviously

dyeing iwas tasteless in interval

and area was asystematic. There was no false flocculus to form. Coloration exponent result indicated that the expression of col-Ⅰ

col-Ⅲ in EXRAR group

RAR group decreased. Conclusion: . RAR and it's extraction has a significant effect on anti-fibrosis

furthermore

RAR's extraction has the same effect.The possible mechanism is related to inhibiting the composition of ECM and encouraging the degradation of ECM.

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