HPLC测定白花蛇舌草配方颗粒中槲皮素和山奈素含量

梁洁; 柳贤福; 孙正伊; 覃日宏; 甄汉深; 翁翎

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HPLC测定白花蛇舌草配方颗粒中槲皮素和山奈素含量

更新时间：2024-01-04

- HPLC测定白花蛇舌草配方颗粒中槲皮素和山奈素含量
- Determination of Quercetin and Kaempferol in Baihua Sheshecao Dispensing Granule by HPLC
- 中国实验方剂学杂志 2012年18卷第24期页码：149-151
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- 纸质出版日期：2012
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梁洁, 柳贤福, 孙正伊, 等. HPLC测定白花蛇舌草配方颗粒中槲皮素和山奈素含量[J]. 中国实验方剂学杂志, 2012,18(24):149-151.

LIANG Jie, LIU Xian-fu, SUN Zheng-yi, et al. Determination of Quercetin and Kaempferol in Baihua Sheshecao Dispensing Granule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(24): 149-151.
梁洁, 柳贤福, 孙正伊, 等. HPLC测定白花蛇舌草配方颗粒中槲皮素和山奈素含量[J]. 中国实验方剂学杂志, 2012,18(24):149-151. DOI：

LIANG Jie, LIU Xian-fu, SUN Zheng-yi, et al. Determination of Quercetin and Kaempferol in Baihua Sheshecao Dispensing Granule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(24): 149-151. DOI：

摘要

目的: 建立测定白花蛇舌草配方颗粒中槲皮素和山奈素含量的HPLC方法。方法: 采用正交试验优化白花蛇舌草配方颗粒的提取工艺

用HPLC测定白花蛇舌配方颗粒中槲皮素和山奈素的含量。色谱柱为Hypersil ODS C18柱(4.6 mm×250 mm

5 μm)

流动相甲醇-0.2%磷酸(45∶55)

检测波长360 nm

流速1.0 mL·min-1

柱温30 ℃。结果: 槲皮素在0.09~0.72 μg线性关系良好(r=0.999 9)

山奈素在0.034 3~0.274 4 μg线性关系良好(r=0.999 9)

槲皮素、山奈素的平均加样回收率分别为100.80%(RSD 1.79%)

101.75%(RSD 1.76%)。结论: 该方法简便、快速、重复性好

可作为白花蛇舌草配方颗粒中槲皮素和山奈素的含量测定。

Abstract

Objective:To establish an HPLC method for determination of quercetin and kaempferol in Baihua Sheshecao dispensing granule. Method: Orthogonal experiments were used to optimize extraction technology. The samples were separated on a Hypersil ODS C18 (4.6 mm×250 mm

5 μm) column which was eluted with methanol-0.2% phosphoric acid solution (45∶55) with detective wavelength at 360 nm and flow rate at 1 mL·min-1

column temperature at 30 ℃. Result: The method had good linear relationship within the range of 0.09-0.72 μg (r=0.999 9) for quercetin and 0.034 3-0.274 4 μg (r=0.999 9) for kaempferol. The average recovery rate were 100.80% (RSD 1.79%) and 101.75% (RSD 1.76%). Conclusion: The method is simple

rapid and reliable which can be used to determine quercetin and kaempferol in Baihuashe Shecao dispensing granule.

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