全蝎-蜈蚣药对对哮喘模型大鼠气道炎症及气道重塑的影响

李海燕; 杨佩兰; 黄海茵; 崔金刚; 汤杰; 吴银根

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全蝎-蜈蚣药对对哮喘模型大鼠气道炎症及气道重塑的影响

更新时间：2024-01-04

- 全蝎-蜈蚣药对对哮喘模型大鼠气道炎症及气道重塑的影响
- Effects of Scorpio-Scolopendra on Expressions of Airway Inflammation and Airway Remodeling in Asthmatic Rats
- 中国实验方剂学杂志 2013年19卷第1期页码：206-210
- 作者机构：
- 作者简介：
- 基金信息：
  
  上海中医药大学附属岳阳中西医结合医院项目(11yj02)
- DOI：
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
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李海燕, 杨佩兰, 黄海茵, 等. 全蝎-蜈蚣药对对哮喘模型大鼠气道炎症及气道重塑的影响[J]. 中国实验方剂学杂志, 2013,19(1):206-210.

LI Hai-yan, YANG Pei-lan, HUANG Hai-yin, et al. Effects of Scorpio-Scolopendra on Expressions of Airway Inflammation and Airway Remodeling in Asthmatic Rats[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(1): 206-210.
李海燕, 杨佩兰, 黄海茵, 等. 全蝎-蜈蚣药对对哮喘模型大鼠气道炎症及气道重塑的影响[J]. 中国实验方剂学杂志, 2013,19(1):206-210. DOI：

LI Hai-yan, YANG Pei-lan, HUANG Hai-yin, et al. Effects of Scorpio-Scolopendra on Expressions of Airway Inflammation and Airway Remodeling in Asthmatic Rats[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(1): 206-210. DOI：

摘要

目的: 观察全蝎-蜈蚣药对对哮喘模型大鼠气道炎症及气道重塑的影响

初探全蝎-蜈蚣治疗哮喘的作用机制。方法: 30只雄性SD大鼠随机分为3组:正常组(A组)、模型组(B组)、全蝎-蜈蚣组(C组)

每组10只。以卵白蛋白致敏并长期吸入激发

制备大鼠支气管哮喘模型。C组予以全蝎-蜈蚣(0.625 g·kg-1·d-1)ig

每天1次

A组、B组则同时予以等量生理盐水ig

治疗3周。检测各组大鼠支气管肺泡灌洗液(BALF)中白细胞(WBC)、嗜酸粒细胞(E)、吞噬细胞(M)、淋巴细胞(L)、中性粒细胞(N)计数及百分比

取支气管肺组织制作病理切片

HE染色、Masson染色观察各组大鼠支气管肺组织病理改变

医学图像分析软件检测支气管壁厚度(Wat/Pbm)、平滑肌厚度(Wam/Pbm)、胶原纤维厚度(Wac/Pbm)

反映气道重塑的程度。结果: 与正常组比较

模型组大鼠BALF中细胞数量、中性粒细胞、淋巴细胞、嗜酸粒细胞比例明显增加(P<0.01)

模型组Wat/Pbm

Wam/Pbm

Wac/Pbm分别为(155.32±39.92)

(22.11±3.42)

(8.47±3.01)μm2/μm

正常组分别为(71.01±11.02)

(5.51±1.76)

(2.37±1.03)μm2/μm

模型组支气管壁、平滑肌层厚度明显增加(P<0.01)、胶原纤维明显增生(P<0.01);与模型组比较

全蝎-蜈蚣组大鼠BALF中细胞数量、中性粒细胞、淋巴细胞、嗜酸粒细胞比例明显减少(P<0.01)

Wat/Pbm

Wam/Pbm

Wac/Pbm分别为(100.67±15.04)

(17.08±4.92)

(4.07±1.08)μm2/μm

支气管壁、平滑肌层厚度明显减轻(P<0.01)、胶原纤维增生程度明显减轻(P<0.01)。结论: "全蝎-蜈蚣"可改善哮喘模型大鼠气道炎症

同时减少支气管壁和平滑肌厚度

减轻胶原纤维增生

对气道重塑有一定的改善或延缓作用。

Abstract

Objective:To observe the impact of Scropio-Scolopendra on airway inflammation and airway remodeling of asthmatic rats

and to explore the mechanism of action of Scropio-Scolopendra for asthma. Method: Thirty SD rats were randomly divided into three groups:normal control group(group A)

model control group(group B)

Scropio-Scolopendra group(group C)

10 in each group.The asthmatic rat airway remodeling model was established by ovalbumin sensitized method

The rats in C group was administrated Scropio-Scolopendra by ig(0.625 g·kg-1·d-1)

and in A

B group rats were administrated the equal physiological saline in the same way. After three weeks of treatment

the number and the percentage of eosinophil(E)

macrophage(M)

lymphocyte(L)

neutrophilic in bronchoalveolar lavage fluid(BALF).Pulmonary pathological changes were observed by HE stain and Masson stain.The thickness of bronchial wall (Wat/Pbm)

smooth muscle(Wam/Pbm) and collagen fibers(Wac/Pbm)were determined in medical image analysis software. Result: :Compared with normal group

number of total leukocytes and the percentage of eosinophil

neutrophil

lymphocyte in BALF were significantly higher in the model group(P<0.01). The thickness of bronchial wall (Wat/Pbm)

smooth muscel layer(Wam/Pbm) and collagen fibers(Wac/Pbm) in model group [(155.32±39.92)

(22.11±3.42)

(8.47±3.01) μm2/μm] were all significantly increased than those in normal group [(71.01±11.02)

(5.51±1.76)

(2.37±1.03) μm2/μm](P<0.01). Compared with model group

number of total leukocytes and the percentage of eosinophil

neutrophil

lymphocyte in BALF were significantly lower in Scropio-Scolopendra group [(100.67±15.04)

(17.08±4.92)

(4.07±1.08) μm2/μm](P<0.01).The thickness of bronchial wall (Wat/Pbm)

smooth muscel layer(Wam/Pbm) and collagen fibers(Wac/Pbm) were significantly decreased in Scropio-Scolopendra group (P<0.01). Conclusion: Scropio-Scolopendra can reduce airway inflammation of asthmatic rats

and play an inhibitory role in airway remodeling by reducing the bronchial wall and smooth muscle thickness

the proliferation of collagen fibers.

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