Objective:To observe the effect of Huoxue Jiedu formula on angiotensin Ⅱ(Ang Ⅱ) and endothelin-1(ET-1) in retina of diabetic rats. Method: A single intraperitoneal injection of streptozotocin(65 mg·kg-1)was used to induce diabetic rats. Model rats were randomly divided into diabetes mellitus(DM)group

Huoxue Jiedu formula groups(15.4

7.70

3.85 g·kg-1) and Doxium group(0.167 g·kg-1).The normal group and model group were given normal water by intragastric administration. All rats were killed after 20 weeks. The serum AngⅡ and ET-1 levels were measured. Immunological histochemistry assay was used to observe the express of ET-1.The expressive abundance of ET-1mRNA in rat's retina were detected by real-time quantitative PCR. Result: The levels of AngⅡ and ET-1 in serum of model group were (417.95±156.11)

(330.12±81.59) ng·L-1

which was higher than control group.The retinal ET-1 protein expression and ET-1mRNA were significantly higher in DM rats than in control group(P＜0.01).The levels of ET-1 in serum of Huoxue Jiedu formula groups were lower than those in model group(P＜0.01).Compared with the model group

the expression of ET-1 in Huoxue Jiedu formula groups depressed evidently(P＜0.01)and the expression of ET-1mRNA were reduced than those in model groups(P＜0.01). Conclusion: Huoxue Jiedu formula medicine can reduce the expression of ET-1 in retina of diabetic rats.