Objective:To establish the method for determining simultaneously four components in Sijunzi Tang

ginsenosid Rg1

ginsenosid Re

ginsenosid Rb1 and glycyrrhizic acid. Method: HPLC was carried out on the Hypersil BDS column(4.6 mm× 250 mm

5 μm) with acetonitrile-0.03% phosphoric acid

gradient elution

the volume of flow 0.9 mL·min-1

column temperature 30 ℃

detection wavelength 202 nm and 250 nm. Result: The correlation coefficients of the four components were higher than 0.999 and the average recoveries were 100.86%

100.57%

97.88%

99.25%

respectively;and RSD of the above four components were 2.34%

2.95%

2.86%

2.97%

respectively. Conclusion: The method is proved to be so easy and accurate and practical that it can be used to determine the four components in Sijunzi Tang.