Objective:To develop a HPLC method for determination of the content of luteolin and diosmetin in Buchnera cruciata. Method: Samples were extracted for 90 minutes with methanol

a Agilent Extend-C18 column(4.6 mm×250 mm

5 μm) was used for composition separation and the mobile phase consisted of acetonitrile-0.7%acetic acid (30∶70) with a flow rate of 1.0mL·min-1.The detection wavelength was at 350 nm

column temperature was at 35 ℃. Sample volume is 10 μL

The content of luteolin and diosmetin in B. cruciata was determined by external standard method. Result: The linear of luteolin and diosmetin concentrations were within 1.126-112.6 mg·L-1(r=0.999 7)

0.886-88.6μg·ml-1(r=0.999 5)

the average recoveries of luteolin and diosmetin were 101.3%(RSD 1.2%

n=6)

99.4%(RSD 2.2%

n=6). Conclusion: The method is simple

accurate and reproducible with good stability

can be used as quality control methods for B. cruciata medicinal materials.