Objective:To research the effect of polysaccharides of Radix Rosa laevigata and Caulis Rosa laevigata on intestinal flora of mouse. Method: Intestinal microbe dysbiosis in mice were constructed by intragastric administration of lincomycin (15 g·kg-1

bid

3 d). The mice were given polysaccharides of Radix Rosa laevigata and Caulis Rosa laevigata (100 g·kg-1 of crude drug for 6 days

) and then normal intestinal flora of fresh fecal was taken on the 10th day to observe the changes in normal intestinal microflora in the first 10 days. The result was expressed as lg10n·g-1.The nomal control

positive control and spontaneous recovery groups were set up. Result: Intestinal microbe dysbiosis in mice was successfully established. After the intestinal microbial flora in mice was disturbed and then treatment by polysaccharides of Radix Rosa laevigata and Caulis Rosa laevigata for 6 days

alteration of intestinal flora in polysaccharides of Radix Rosa laevigata was basically regulated. For polysaccharides of Caulis Rosae laevigatae

comparing with the modle groups

only the quantity of Enterobacteriaceae and Bifidobacterrium was increased significantly (P<0.01). Conclusion: The polysaccharides of Radix Rosa laevigata and Caulis Rosa laevigata have the function of regulating alteration of intestinal flora. Comparing with Caulis Rosae laevigatae

Radix Rosa laevigata has the better curative effect.