Objective:To investigate the change of immunologic function in deficiency cold model induced by hydrocortisone. Method: Forty eight male SD rats were divided into two groups. The control group was intramuscularly injected saline 0.25 mL

the experimental group was intramuscularly injected hydrocortisone sodium succinate saline 20 mg·kg-1. 10 μL blood sample was collected from tail on 7th day

14th day and 21th day.WBC count was detected by the microscope. 5 mL blood sample was collected from abdominal main vein on 15th day and 22th day. Serum IgG and IgM levels were detected by automatic biochemical analyzer. Plasma interleukin-4(IL-4) and interferon-γ(IFN-γ) levels were determinated by elisa. Then thymus and spleen were removed and weighed to calculate the organ index. Result: Compared with the control group

the thymus index and serum IgG of experimental group were significantly lower on 14th day and 21th day(P<0.01

P<0.05)

the spleen index and serum IgM were significantly lower on 14th day(P<0.05)and no change on 21th day

the WBC count were significantly lower on 7th day

14th day and 21th day(P<0.01

P<0.05)

plasma IL-4 was low(P<0.05)

IFN-γ level was high(P<0.05) and IFN-γ/ IL-4 was high(P<0.05)on 21th day. Conclusion: During the experimental process

the immunologic function was significantly lower

and associated with shift of Th1/Th2 in deficiency cold status.