Objective:To screen and establish the experimental animal model of simultaneous liver and kidney lesion in mice

and to provide experimental animal models for screening drugs which used to cure kidney and liver damage. Method: We investigated the damage effect of concanavalin A (Con A)

D-galactosamine (D-Gal) and carbon tetrachloride (CCl4) in the mouse liver and kidney

and we established the effective modeling method by determing the biochemical indicators and the histopathological changes related to the liver and kidney function. To further inspect if the model is able to effectively evaluate the efficacy of drugs for kidney and liver damage

we study the protective effect of Dahuang Gancao decoction on liver and kidney damage using the established model. Result: 1 g·kg-1 D-Gal

CCl4(60 mg·kg-1) could induce liver injury in mice

but there is no evident about the renal injury; 20 mg·kg-1 Con A could induce liver and kidney damage simultaneously

and we determine the best dose of Con A to built simultaneous liver and kidner damage model as 15 mg·kg-1 after further optimization. The biochemical index and histological examination of kidney and liver damage mice were significantly improved after giving the prescription Dahuang Gancao decoction. Conclusion: We initially established the experimental model of simultaneous liver and kidney damage by injecting Con A 15 mg·kg-1 through tail vein

and proved to be successful by verification of Dahuang Gancao docoction.