Objective:To investigate the effects of Buyang Huanwu decocotion combined with Liuwei Dihuang Wan on learning memory

amyloid precursor protein (APP)

growth associated protein 43 (GAP-43) and synaptophysin SYP expression in permanent cerebral ischemia model in rats. Method: To establish the permanent middle cerebral artery model in rats. Male SD rats were randomly divided into five groups: normal group

model group

Buyang Huanwu decocotion group

Liuwei Dihuang Wan group and combination group. After treatment for 30 days

Morris water maze was used to measure the learning memory

and the technology of Western blot was adopted to examine the expression of APP

SYN

GAP-43. Result: After the ischemia model was established for 30 days

the spatial memory was damaged obviously in model group. Buyang Huanwu decoction and Liuwei Dihuang Wan as well as the combination therapy could improve the spatial memory ability in different degrees. The escape latencies of the combination decoction were decreased significantly compared to the model group (P<0.05) on the second day of position navigation test. On the fourth day of position navigation test

the escape latencies in Buyang Huanwu decocotion group and the combination decoction group was decreased obviously (P<0.05). In the spatial probe testing

the piercing number in Buyang Huanwu decocotion group and the combination decoction group were increased significantly compared to the model group

the first time through the original plat of the combination decoction group was shorted remarkably compared to the model group (P<0.01). Compared to sham group

the APP expression of the model group was increased significantly and the GAP-43 and SYN expression was descended obviously (P<0.01

P<0.05) while Buyang Huanwu decoction group and the combination decoction could decrease the APP expression and increase the GAP-43

SYN expression remarkably compared to the model group (P<0.05). There was no statistical significance inj the APP expression between Liuwei Dihuang Wan group and model group. The GAP-43 and SYN expression was increased obviously (P<0.05). Conclusion: Buyang Huanwu decoction combined with Liuwei Dihuang Wan can alleviate the cerebral ischemia injury and reduce the abnormal APP accumulation. In addition

the combination decoction can reduce GAP-43 and SYN synthesis and promote regeneration of neurite

and promote the recovery of learning memory.