不同型号大孔树脂对金荞麦中有效成分的纯化工艺考察

盛华刚; 朱立俏

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不同型号大孔树脂对金荞麦中有效成分的纯化工艺考察

更新时间：2024-01-04

- 不同型号大孔树脂对金荞麦中有效成分的纯化工艺考察
- Investigation of Purification Technology for Effective Constituents from by Different Types of Macroporous Resin
- 中国实验方剂学杂志 2012年18卷第24期页码：41-44
- 作者机构：
- 作者简介：
- 基金信息：
  
  山东省高等学校科技计划项目(J11LF30)
- DOI：
  中图分类号：
- 纸质出版日期：2012
- 稿件说明：
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盛华刚, 朱立俏. 不同型号大孔树脂对金荞麦中有效成分的纯化工艺考察[J]. 中国实验方剂学杂志, 2012,18(24):41-44.

SHENG Hua-gang, ZHU Li-qiao. Investigation of Purification Technology for Effective Constituents from by Different Types of Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(24): 41-44.
盛华刚, 朱立俏. 不同型号大孔树脂对金荞麦中有效成分的纯化工艺考察[J]. 中国实验方剂学杂志, 2012,18(24):41-44. DOI：

SHENG Hua-gang, ZHU Li-qiao. Investigation of Purification Technology for Effective Constituents from by Different Types of Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(24): 41-44. DOI：

摘要

目的: 考察不同型号大孔树脂对金荞麦中有效成分的纯化效果。方法: 以表儿茶素和金E的含量、浸膏率为指标

结合HPLC指纹图谱

选择最佳型号大孔树脂。结果: AB-8型大孔树脂对表儿茶素洗脱率为89.78%

对金E洗脱率为93.04%。HPLC指纹图谱共有峰面积比较表明AB-8型大孔树脂最适合用于金荞麦有效成分的纯化。结论: 采用AB-8型大孔树脂纯化金荞麦中有效成分是可行的

且操作简单、稳定。

Abstract

Objective:To investigate purification effect of different types of macroporous resin on effective constituents from Fagopyrum dibotrys. Method: With the content of epicatechin and mixture of proanthocyanidins tannic condensation

extract rate as indexes

optimum resin was selected by combining with HPLC fingerprint. Result: Elution rate of epicatechin by AB-8 resin was 89.78%

elution rate of mixture of proanthocyanidins tannic condensation by AB-8 resin was 93.04%.Comparison of the common peak area of HPLC fingerprint showed that AB-8 resin was the most suitable one to purify effective constituents from F. dibotrys. Conclusion: This process of applying AB-8 resin to purity effective constituents from F. dibotrys was feasible

simple and stable and could be extended to large-scale production applications.

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