Objective: To observe the cardioprotective effects of total organic acids in Fructus Choerospondiatis on myocardial ischemia-reperfusion injury. Method: In vivo experiments

rat were given total organic acids in Fructus Choerospondiatis 0.5 g·kg-1 by intragastric administration

once per day and for 3 days. One hour after the last administration

rats underwent 40 min of left coronary artery occlusion followed by 120 min reperfusion. The myocardial infarct size was measured by N-BT staining. The coagulation parameters such as prothrombin time (PT)

partial thromboplastin time (APTT)

clotting time (TT)

fibrinogen (Fib) were measured. In vitro experiments

the protective effect of total organic acids in Fructus Choerospondiatis was observed on cardiomyocytes hypoxia/reoxygenation injury and the intensity of protective effects of single organic acids was compared. Result: ① Total organic acids in Fructus Choerospondiatis 0.5 g·kg-1 could significantly reduce myocardial infarct size and weight

and reduce the percentage of infarct myocardium in ventricular (heart)s size(P<0.01). Total organic acids in Fructus Choerospondiatis 0.5 g·kg-1 had tendency to reduce the contents of plasm PT

TT and increase the content of plasma Fib

but there were no significant difference. ② Total organic acids in Fructus Choerospondiatis 400 mg·L-1 significantly decreased LDH release rate of cardiomyocytes injured by hypoxia/reoxygenation (P<0.01). ③ Citric acid

L-malic acid

succinic acid and tartaric acid 400 mg·L-1 significantly decreased LDH release rate of cardiomyocytes injured by hypoxia/reoxygenation

with the effect of citric acid was more obvious. Conclusion: Total organic acids in Fructus Choerospondiatis have protective effects on acute myocardial ischemia-reperfusion. Citric acid

L-malic acid

succinic acid and tartaric acid may be the partial substance basis of Fructus Choerospondiatis on myocardial ischemia-reperfusion injury.