Objective: To establish a method for the determination of salvianolic acid A in the plasma samples

and then study the plasma protein binding rate of salvianolic acid A. This can provide a reference to the further study of salvianol acid A in its metabolism

pharmacokinetics and clinical treatment. Method: The ultrafiltration was employed to determine the plasma protein binding rate of salvianol acid A in BSA plasma samples

rat plasma samples

the New Zealand rabbit plasma samples and beagle dog plasma samples. The plasma concentrations of salvianol acid A were measured by RP-HPLC. Result: The calibration curve of salvianolic acid A was linear within the ranges of 0.01-2.5 mg· L-1(r=0.999 4).The average plasma protein binding rates of salvianol acid A with BSA

rat plasma

the New Zealand rabbit plasma and beagle dog plasma were (99.79±0.02)%

(99.79±0.03)%

(99.73±0.06)%

(99.81±0.03)% in the plasma concentrations of 5.0

50.0

100.0 mg· L-1 respectively. Conclusion: The method has high sensitivity

good specificity and reproduction

with simple management thus fulfilling the requirement. Salvianol acid A shows a high binding power to plasma protein.

and this was independent of the investigated concentrations and the different species.