Objective:To describe a method for determining of salidroside and paeoniflorin in compound Qitian capsules by HPLC. Method: The determination was carried out over a COSMOSIL column (4.6 mm×250 mm

5 μm) at the temperature (35℃). The mobile phase consisted of methanol (A)-0.02％ phosphoric (B) solution with the gradient elution (0-15 min

19%; 15-50 min

28%A; 50-60 min

28%A

the flow rate:1 mL·min-1 and the detection wavelength was set at 275 nm during 0-25 min and 230 nm during 26-60 min. Result: Two regression equation showed good linear relationship between the peak area ratio of each marker to internal standard and amounts. The average recovery of salidroside was 98.33% (RSD 0.71%)

of paeoniflorin was 97.77% (RSD 1.18%). Conclusion: The method is simple

accurate

sensitive

and can be used for determination of two components in Qitian capsules with satisfactory accuracy and repeatability.