Objective:To study the time-effect of serum containing the formula of Tanyu Tongzhi (TYTZ). Method: Serum containing TYTZ was obtained in 2 ways: administration was twice and once

respectively

a day or for 5 days. The blood was collected at 0.5

1

1.5

2

3 h after last administration. The influence of the serum containing TYTZ on cell proliferation was detected with MTT colorimetric method

the stromal cell-derived factor-1(SDF-1)content in the different serum was detected with the kit. Result: Compared with blank serum

the serum containing medicine could promote cell proliferation

which showed time-dependent. There was significant difference in SDF-1 content between the serum containing medicine and blank serum. While there was no significant difference in cell proliferation between the two ways of obtaining serum. And there was a significant difference in SDF-1 content between the two ways. Conclusion: In the conditions of this experiment

preparing serum need not to be constrained to the 'general principles’

but depends on the specific experiment.