Objective:To study on optimum separation and purification technology conditions of genidenoside from extraction liquid of Gardenia jasminoides by macroporous resin. Method: With adsorption-elution volume

adsorption-elution rate as indexes

eight kinds of macroporous resins were selected by adsorption and desorption test

optimum separation and purification technology conditions of genidenoside was investigated by single factor test of static-dynamaic adsorption and desorption. Result: X-5 type macroporous resin had the best effect of separation and purification

its optimum separation and purification technology conditions were:the concentration of sample liquid 20.00 g·L-1

temperature 25 ℃

pH 5.0

eluent of 60% ethanol

flow rate of dynamaic adsorption 2.0 BV·h-1

sample solution volume 3.0 BV

flow rate of dynamaic desorption 3.0 BV·h-1

eluent volume 7.5 BV. Conclusion: This optimized method was feasible and reliable

it should be widely applied.