首乌方与美多芭同用对PD模型大鼠纹状体细胞外液神经化学物质的影响

牛晓红; 王丹巧; 孙晓芳; 吴兆恩; 李鹏; 王巍

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首乌方与美多芭同用对PD模型大鼠纹状体细胞外液神经化学物质的影响

更新时间：2024-09-23

- 首乌方与美多芭同用对PD模型大鼠纹状体细胞外液神经化学物质的影响
- Effects of Shouwu Fang and Madopar Simultaneous on Neurochemical Substances of Striatal Extracellular Fluid in Parkinson's Disease Rats
- 中国实验方剂学杂志 2013年19卷第3期页码：155-159
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目(30772780)
- DOI：
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
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牛晓红, 王丹巧, 孙晓芳, 等. 首乌方与美多芭同用对PD模型大鼠纹状体细胞外液神经化学物质的影响[J]. 中国实验方剂学杂志, 2013,19(3):155-159.

NIU Xiao-hong, WANG Dan-qiao, SUN Xiao-fang, et al. Effects of Shouwu Fang and Madopar Simultaneous on Neurochemical Substances of Striatal Extracellular Fluid in Parkinson's Disease Rats[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 155-159.
牛晓红, 王丹巧, 孙晓芳, 等. 首乌方与美多芭同用对PD模型大鼠纹状体细胞外液神经化学物质的影响[J]. 中国实验方剂学杂志, 2013,19(3):155-159. DOI：

NIU Xiao-hong, WANG Dan-qiao, SUN Xiao-fang, et al. Effects of Shouwu Fang and Madopar Simultaneous on Neurochemical Substances of Striatal Extracellular Fluid in Parkinson's Disease Rats[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 155-159. DOI：

摘要

目的: 应用清醒自由活动大鼠脑内微透析及其相关检测技术

探讨首乌方与美多芭同时应用对帕金森病(PD)模型大鼠纹状体细胞外液神经化学物质的影响。方法: SD大鼠

随机分为对照组、模型组、美多芭组(30 mg·kg-1)、首乌方+美多芭组(含首乌方生药量18 g·kg-1+美多芭30 mg·kg-1)。单侧纹状体内注射6-羟基多巴胺造模

清醒自由活动状态下脑内纹状体微透析采样

高效液相-电化学检测样品中多巴胺(DA)及其代谢产物高香草酸(HVA)、二羟基苯乙酸(DOPAC)、羟自由基指标(2

3DHBA

5DHBA)浓度。结果: 与美多芭组相比

首乌方+美多芭组纹状体细胞外液DA水平升高

最高达(39.15±28.70) μg·L-1

DA变化率(ΔDA)降低;羟自由基指标在观察时间内始终低于美多芭组

有9个时间点P<0.05。结论: 首乌方可增加纹状体细胞外液DA递质的浓度并减少其波动;抑制羟自由基产生

对神经细胞具有保护作用。

Abstract

Objective:To study the synergic effects of Shouwu Fang and Madopar on striatal neurochemical substances in freely moving rats with Parkinson's disease (PD) by brain microdialysis and its relevant detection technology. Method: SD rats were randomly divided into control group

model group

Madopar group

Shouwu plus Madopar group.Parkinson's disease animal model was induced by injection of 6-hydrodopamine (6-OHDA) into striatum of SD rats. Brain microdialysis was performed and concentrations of dopamine (DA) and its metabolites homovallinic acid (metabolic protact of DA)

dihydroxyphenylacetic acid (DOPAC)

3-dihydroxy benzyl acid and 2

5-dihydroxy benzyl acid (the index of hydroxyl radical) were detected by high performance liquid chromatography-electrochemical detector (HPLC-ED). Result: Compared with Madopar group

Shouwu Fang plus Madopar group treatment elevated extracellular DA level in striatum with peak at (39.15±28.70) μg·L-1 as well as reduced the fluctuation of DA (ΔDA). In addition

during whole observation period

hydroxyl free radical generation in Shouwu Fang plus Madopar group was significantly lower than Madopar group (with 9 time points P<0.05). Conclusion: Shouwu Fang could raise DA concentration and reduce its fluctuation in extracelluar fluids of straitum. It could also inhibit the generation of hydroxyl free radical and protect neurons from damage under oxidative stress.

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