RP-HPLC同时测定四逆汤中3种双酯型乌头碱的含量

刘晓; 范林乾; 蔡皓; 束雅春; 蔡宝昌

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RP-HPLC同时测定四逆汤中3种双酯型乌头碱的含量

更新时间：2024-01-04

- RP-HPLC同时测定四逆汤中3种双酯型乌头碱的含量
- Simultaneous Determination of Aconitine, Hypaconitine and Mesaconitine in Sini Decoction by RP-HPLC
- 中国实验方剂学杂志 2012年18卷第22期页码：105-108
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家中医药管理局中医药行业科研专项(201007010)
- DOI：
  中图分类号：
- 纸质出版日期：2012
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刘晓, 范林乾, 蔡皓, 等. RP-HPLC同时测定四逆汤中3种双酯型乌头碱的含量[J]. 中国实验方剂学杂志, 2012,18(22):105-108.

LIU Xiao, FAN Lin-qian, CAI Hao, et al. Simultaneous Determination of Aconitine, Hypaconitine and Mesaconitine in Sini Decoction by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(22): 105-108.
刘晓, 范林乾, 蔡皓, 等. RP-HPLC同时测定四逆汤中3种双酯型乌头碱的含量[J]. 中国实验方剂学杂志, 2012,18(22):105-108. DOI：

LIU Xiao, FAN Lin-qian, CAI Hao, et al. Simultaneous Determination of Aconitine, Hypaconitine and Mesaconitine in Sini Decoction by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(22): 105-108. DOI：

摘要

目的: 建立RP-HPLC同时测定四逆汤中乌头碱、次乌头碱、新乌头碱3种成分的含量。方法: 采用Thermo Hypersil BDS (4.6 mm×250 mm

5 μm) 色谱柱

流动相甲醇-0.2%三乙胺水溶液(冰乙酸调pH 5.2)(46∶54)

流速1 mL·min-1

检测波长235 nm

柱温25 ℃。结果: 在35 min内乌头碱、次乌头碱和新乌头碱分别达到良好分离;依次在0.875~28 mg·L-1 (r=0.999 8)

1.125~36 mg·L-1 (r=0.999 9)和0.812~26 mg·L-1 (r=0.999 8)呈良好的线性关系;3种成分的加样回收率分别为94.9%

100.2%

99.9%。结论: 方法简单可行

适用于四逆汤中乌头碱、次乌头碱和新乌头碱3种双酯型乌头碱成分的同时含量测定。

Abstract

Objective:To establish an RP-HPLC method for simultaneous determination of aconitine

hypaconitine and mesaconitine in Sini decoction. Method: Hypersil C18 was used as chromatographic separation column

and methanol-0.2% triethylamine (pH adjusted to 5.3 with CH3COOH) was used as the mobile phase. The detection wavelength was set at 230nm. The flow rate was 1.0 mL·min-1 and the column temperature was set at 25 ℃. Result: Aconitine

hypaconitine and mesaconitine were linear in the range of 0.875-28 mg·L-1 (r=0.999 8)

1.125-36 mg·L-1 (r=0.999 9) and 0.812-26 mg·L-1 (r=0.999 8)

respectively. Their average recoveries were 94.9% for aconitine

100.2% for hypaconitine and 99.9% for mesaconitine

respectively. Conclusion: The established method is convenient and can be helpful for the quality control of Sini decoction.

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