Objective: To establish the fingerprint chromatograms of Flos Gentianae Stramienae by the means of liquid chromatography-mass spectrography (LC-MS). Method: The analysis was performed on Agilent ZORBAX ECLIPSE XDB-C18 column (2.1 mm×50 mm

1.8 μm) with the mobile phase consisting of 0.1% formic acid-water-acetonitrile in a gradient mode. The chromatograms were recorded by using ESI-MS in positive ion model. Totally

11 batches of samples were analyzed and their similarities were calculated using fingerprint similarity evaluation software. Result: The LC-MS fingerprint common mode of Flos Gentianae Stramienae was obtained. A total of 23 characteristic peaks were selected as the common fingerprint peaks. The similarities of 11 samples were greater than 0.90 as compared to the common mode and seven peaks were identified by using HPLC-ESI-Q-TOF-MS. Conclusion: The reported method is precise

reproducible and reliable. The establishment of LC-MS fingerprint chromatograms can be helpful for the quality control of Flos Gentianae Stramienae.