Objective: To choose the optimal extraction conditions of Rhizoma Atractylodis and Cortex Magnolioe Officinalis compatibility by supercritical CO2 and control the quality of the extract. Method: The orthogonal design experiments were used to choose the optimal conditions and the factors were extraction temperature

extraction pressure

resolution temperature and resolution pressure. The extraction rate was used as inspection index. The essaying by HPLC was established. Result: The optimal conditions were as follows:extraction temperature at 50℃

extraction pressure of 26 MPa

resolution temperature of 35℃

resolution pressure of 8 MPa. The average content of honokiol

magnolol and atisine chloride atractydin was 9.81%

12.52%

7.26%. Conclusion: The method of supercritical CO2 extraction is reliable and feasible; it can be used in industry. The quality of extract is stable and controlled.