Objective:To determine the content of total flavonoids in several different sources of Gaoziban. Method: Using rutin as a reference substance

the content of total flavonoids in Gaoziban of Asperugo procumbens from Hetian

Cele

and Yutian

Anchusa italica

Borage officinales

and Cynoglossum officinales were determined at 510 nm by UV spectrophotometry colored with sodium nitrite-aluminum nitrate-sodium hydroxide. Result: Content of total flavonoids in three Gaozibans of Anchusa italica

Borage officinales

Cynoglossum officinales were 3.85

3.72

2.35 mg·g-1; and three Gaozibans of A. procumbens from Hetian

Cele and Yutian were 3.14

4.73

3.67 mg·g-1 respectively

among which the content of total flavonoids in Gaoziban was highest in A. procumbents from Cele and lowest in C. officinales. Conclusion: The method was accurate

reliable

repeatable

and could be used to determine the content of total flavonoids in Gaoziban. The content of total flavonoids is not much different from A. italica

B. officinales and A. procumbens.