Objective:To investigate the effect of N-acetylcysteine (NAC) on acute injured kidney (AKI) induced by cisplatin (CDDP). Method:The rats were injected CDDP intravenously to make AKI model. The rats were randomly divided into 6 groups

including the normal control group

CDDP-induced AKI model group

NAC groups (50

100

200 mg·kg-1) and Vit C control group (100 mg·kg-1). NAC and Vit C were administered once a day three days before CDDP was given. And then NAC and Vit C were continuously given ip for five days. The body weight

kidney weight

kidney index

urinary protein excretion rate of rats were determined. The commercial kits were employed to test the concentrations of lactate dehydrogenase (LDH)

malondialdehyde (MDA)

superoxide dismutase (SOD)

catalase (CAT)

reduced glutathione (GSH)

glutathione peroxidase (GSH-Px)

15-F2t-isoprostane (15-F2t-Isop). Eight-OHdG positive cells were counted by immunohistochemical staining. The expression of Cu-Zn-SOD was measured by Western blot. Result:Compared with the normal control group

the weight of body and kidney

kidney index

the concentration of CAT

SOD

GSH

GSH-Px and Cu-Zn-SOD expression were significantly decreased

while urinary protein excretion rate and the concentration of LDH

MDA

15-F2t-Isop

8-OHdG positive cells were significantly increased (P<0.01) in the CDDP-induced kidney injury group. NAC and Vit C significantly reduced urinary protein excretion rate and the concentration of LDH

MDA

15-F2t-Isop

8-OHdG positive cells and increased kidney index

the concentration of CAT

SOD

GSH

GSH-Px and Cu-Zn-SOD expression compared with the AKI model group (P<0.01). Conclusion:NAC protect kidney from CDDP injury by suppressing the oxidative stress.