大孔吸附树脂法优选荔枝草总黄酮纯化工艺

程建明; 葛婷婷; 彭国平; 郑云枫

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大孔吸附树脂法优选荔枝草总黄酮纯化工艺

更新时间：2024-01-04

- 大孔吸附树脂法优选荔枝草总黄酮纯化工艺
- Optimization of Purification Technology for Total Flavonoids from by Macroporous Adsorption Resin
- 中国实验方剂学杂志 2012年18卷第19期页码：63-65
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- 纸质出版日期：2012
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程建明, 葛婷婷, 彭国平, 等. 大孔吸附树脂法优选荔枝草总黄酮纯化工艺[J]. 中国实验方剂学杂志, 2012,18(19):63-65.

CHENG Jian-ming, GE Ting-ting, PENG Guo-ping, et al. Optimization of Purification Technology for Total Flavonoids from by Macroporous Adsorption Resin[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(19): 63-65.
程建明, 葛婷婷, 彭国平, 等. 大孔吸附树脂法优选荔枝草总黄酮纯化工艺[J]. 中国实验方剂学杂志, 2012,18(19):63-65. DOI：

CHENG Jian-ming, GE Ting-ting, PENG Guo-ping, et al. Optimization of Purification Technology for Total Flavonoids from by Macroporous Adsorption Resin[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(19): 63-65. DOI：

摘要

目的:优选荔枝草总黄酮的富集纯化工艺。方法:以总黄酮吸附率、解吸率和转移率为评价指标

采用静态吸附-解吸附法

筛选大孔树脂型号;以总黄酮含量为指标

确定洗脱剂浓度;以除杂率、高车前苷转移率和总黄酮转移率为指标

优选其纯化工艺。结果:D101型树脂分离效果较好

其最佳工艺为生药量-树脂体积1g·mL-1

用7BV75%乙醇溶液洗脱

洗脱流速6mL·min-1。结论:D101树脂可有效地对荔枝草总黄酮进行富集纯化。

Abstract

Objective:To optimize enrichment and purification technology of total flavonoids from Salvia plebeia. Method:With adsorption-desorption ratio and transfer ratio as indexes

optimum macroporous resin was selected by static adsorption-desorption ratio tests;The concentration of eluting agent was determined with the content of total flavonoids as index;Purification technology was optimized with impurity rate

transfer rate of homoplantaginin and transfer rate of total flavonoids as indexes. Result:D101 was selected for its excellent adsorption and desorption properties

optimum process was as follows:the amount of crude drugs to D101 macroporous resin 1 g·mL-1

eluted with 7 BV 75% ethanol

elution flow rate 6 mL·min-1. Conclusion:Total flavonoids from S. plebeia could be effectively purificated and separated by D101 macroporous resin.

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