Objective:To establish an HPLC-ELSD method for simultaneous quantitation of six triterpene acids from Eriobotrya japonica. Method: Samples were analyzed with Ultimate XB C18 column (4.6 mm×250 mm

5 μm) and eluted with acetonitrile-methanol-0.5% ammonium acetate solution as mobile phases in gradient mode

flow rate was 1.0 mL·min-1

column temperature of 35℃

drift tube temperature of ELSD was set at 95℃

and flow rate of nitrogen was 2.5 L·min-1. Result: There were good linear relationship between natural logarithm of peak areas and injection quality at the range of 0.436-2.180

0.392-1.960

0.39-1.960

0.856-4.280

0.424-2.120

1.592-7.960 μg for rosolic acid

tormentic acid

maslinic acid

corosolic acid

oleanolic acid

ursolic acid

respectively.The average recoveries of these six triterpene acids were 98.9%(RSD 3.8%)

96.3%(RSD 2.7%)

97.7%(RSD 2.6%)

97.6%(RSD 3.2%)

96.6%(RSD 1.9%)

102.5%(RSD 2.5%)

respectively. Conclusion: This established method was simple

accurate and repeatable with good separation

it could provide experimental basis for a better quality control of E. japonica.