Objective:To study the expectorant

analgesic and antibacterial effects of Yanshuning granules. Method: Mice were divided into 6 groups randomly: normal control group

positive groups

high dose group

middle dose group and low dose group of Yanshuning granules (11.00

5.50

2.75 g·kg-1)

after 7 d by ig administration

the expectorant effect was studied by a mouse model of phenol red test. The analgesic effect was studied by mouse models of hot-plate induced pain and acetic acid-induced writhing reaction.Experimental research on antibacterial action in vitro of Yanshuning granules was applied to observe the antibacterial effect. Result: The experimental results showed that Yanshuning granules could significantly increase phenol red excretion in mice (P<0.05 or P<0.01). It could significantly prolong the latent time of reaction on hot-plate in mice(P<0.05)

reduce the writhing times in mice(P<0.05 or P<0.01).The granules showed obvious antibacterial actions against Staphylococcus aureus[minimal inhibitory loncentration(MIC) is 0.250 g·mL-1

minimal bactericidal concentration(MBC) is 0.500 g·mL-1]

A-hemolytic streptococcus(MIC is 0.062 g·mL-1

MBC is 0.250 g·mL-1)

Beta hemolytic streptococcus(MIC is 0.031 g·mL-1

MBC is 0.062 g·mL-1)

Proteus(MIC is 0.125 g·mL-1

MBC is 0.250 g·mL-1) and Streptococcus pneumoniae(MIC is 0.125 g·mL-1

MBC is 0.500 g·mL-1). Conclusion: The granules has obvious expectorant

analgesic and antibacterial effects.