Objective:To screen microbial with attenuated efficiency for Aconitum carmichaeli by solid-state fermentation

and preliminary explore its biotransformation mechanism. Method: With A. carmichaeli powder as raw material

bacteria growth

the content of diester alkaloid and material composition changes as evaluation indexes

suitable bacteria for biotransformation of A. carmichaeli was screened by solid-state fermentation.Structure of new substances were preliminary identified by HPLC-MS

and in order to provide a theoretical basis for biotransformation feasibility of A. carmichaeli. Result: Biotransformation with aspergillus could reduce the content of aconitine

and added a new compound of benzoylaconitine with less toxic. Conclusion: Benzoylaconitine could achieve purpose of attenuated efficiency for A. carmichaeli

which provided a theoretical basis and feasibility to depth study of new processing method for A. carmichaeli.