HPLC测定鹰嘴豆芽素A2种脂质纳米粒包封率和载药量

王强; 程海林; 郭丽丽; 罗晴; 黄霞; 陈卫东

您当前的位置：

首页 >

文章列表页 >

HPLC测定鹰嘴豆芽素A2种脂质纳米粒包封率和载药量

更新时间：2024-01-04

- HPLC测定鹰嘴豆芽素A2种脂质纳米粒包封率和载药量
- Determination of the Entrapment Efficiency and Drug Loading Capacity of Two Kinds of Biochanin A Loaded Lipid Nanoparticles
- 中国实验方剂学杂志 2012年18卷第21期页码：131-136
- 作者机构：
- 作者简介：
- 基金信息：
  
  2011年安徽中医学院大学生"药苑之星"科研基金项目(201111);2012年安徽中医学院大学生科研基金项目(201207)
- DOI：
  中图分类号：
- 纸质出版日期：2012
- 稿件说明：
移动端阅览
王强, 程海林, 郭丽丽, 等. HPLC测定鹰嘴豆芽素A2种脂质纳米粒包封率和载药量[J]. 中国实验方剂学杂志, 2012,18(21):131-136.

WANG Qiang, CHENG Hai-lin, GUO Li-li, et al. Determination of the Entrapment Efficiency and Drug Loading Capacity of Two Kinds of Biochanin A Loaded Lipid Nanoparticles[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(21): 131-136.
王强, 程海林, 郭丽丽, 等. HPLC测定鹰嘴豆芽素A2种脂质纳米粒包封率和载药量[J]. 中国实验方剂学杂志, 2012,18(21):131-136. DOI：

WANG Qiang, CHENG Hai-lin, GUO Li-li, et al. Determination of the Entrapment Efficiency and Drug Loading Capacity of Two Kinds of Biochanin A Loaded Lipid Nanoparticles[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(21): 131-136. DOI：

摘要

目的: 建立鹰嘴豆芽素A固体脂质纳米粒(BCA-SLN)和纳米脂质载体(BCA-NLC)的包封率和载药量的高效液相色谱测定方法。比较2种纳米粒包封率和载药量的差异。方法: 采用超滤离心法分离游离药物

高效液相色谱法测定药物含量。Cosmosil C18色谱柱(4.6 mm×250 mm

5 μm);柱温30℃;流动相乙腈-0.1%磷酸(60:40);流速1 mL ·min-1;检测波长260 nm。结果: BCA在0.005~5.000 mg ·L-1线性良好(r=1.000)。所制BCA-SLN包封率为(97.15±0.28)%

载药量为(6.38±0.043)%;BCA-NLC包封率为(99.57±0.11)%

载药量为 (6.91±0.044)%。比较2种纳米粒包封率和载药量表明均存在显著性差异(P≤ 0.05)。结论: 采用高效液相色谱法结合超滤离心法测定BCA 2种纳米粒包封率和载药量方法准确、高效

NLC具有更高的包封率和载药量。

Abstract

Objective: To establish a HPLC method to determine the entrapment efficiency (EE) and drug loading (DL) of biochanin A loaded solid lipid nanoparticles (BCA-SLN) and biochanin A loaded nanostructured lipid carriers (BCA-NLC). The distinction between the two kinds of nanoparticles in EE and DL was investigated. Method: Centrifugation ultrafiltration method was used to isolate the free drug. The content of drug was determined by HPLC. The analytical column was Cosmosil C18(4.6 mm×250 mm

5 μm) and column temperature was at 30℃. The mobile phase was acetonitrile-0.1% phosphoric acid (60: 40). The flow rate was 1 mL·min-1. The UV detecting wavelength was set at 260 nm. Result: BCA was linear in the range of 0.005-5.000 g ·L-1 (r=1.000). The EE and DL of BCA-SLN were (97.15±0.28)% and (6.38±0.043)%

respectively. The EE and DL of BCA-NLC were (99.57±0.11)% and (6.91±0.044)%

respectively. There was significant difference between two kinds of nanoparticles in EE (P≤0.05)and DL (P≤0.05). Conclusion: HPLC combined with centrifugation ultrafiltration method to determine the EE and DL of BCA-SLN/BCA-NLC is accurate and efficient. The EE and DL of NLC are higher than SLN.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

齐墩果酸脂质体包封率的测定

盐酸倍他洛尔-蒙脱石脂质体的制备与性能考察

不同载药量姜黄素自纳米乳体外分散后相行为与稳定性的相关性考察

毛冬青饮片指纹图谱及定量分析

铁皮石斛与霍山石斛中甘露糖、葡萄糖及柚皮素的含量比较