Objective:To study the effects of Lingxue Huoxue capsule complete ingredients (LC) and its Liangxue(LX) and Jiedu(JD) components on proliferation

activation and cytokine production of activated T lymphocytes

which simulate the key pathological aspects of psoriasis in vitro

under the guidance of therapeutic 'cooling blood and detoxification’. To investigate the scientific basis of TCM therapeutic principles. Method: Jurkat T cell lines were stimulated with phorbol 12

13-dibutyrate(PDB) and ionomycin(Iono) in vitro to simulate the key pathological aspects of psoriasis. Lingxue Huoxue capsule complete ingredients were divided into LX and JD groups. Serum pharmacological method was used for the experiments. The CCK-8 assay was used for T cell proliferation

flow cytometry was adapted to CD69 expression

and the level of cytokines was measured by enzyme linked immuno sorbent assay (ELISA) in this study. Result: LC and its LX and JD components significantly inhibited the T lymphocyte activities and obviously inhibited CD69 expression

decreased the level of IFN-γ

IL-2 and TNF-α. Among the three groups

LC group was the superior

particularly in cells activities and TNF-α production. The inhibition of JD group was stronger than LX group's

except for the secretions of IL-2 and TNF-α. In addition

the immunosuppression of JD group was close to the effects of LC especially on T cells activities and the level of IFN-γ. Conclusion: LC and its LX and JD components can inhibit proliferation

activation and cytokine production of T lymphocytes

as well as

the effects of the complete prescription of LXHX is superior among the three groups. This suggests that 'heat and toxin coexist in blood’ which is the key point of the pathogenic mechanisms in psoriasis

and cooling blood and detoxicification cannot be separated in clinical treatments.