HPLC同时测定脉络宁注射液中8种主要成分的含量

吴婷; 张军; 周玲; 居文政; 谈恒山

您当前的位置：

首页 >

文章列表页 >

HPLC同时测定脉络宁注射液中8种主要成分的含量

更新时间：2024-01-04

- HPLC同时测定脉络宁注射液中8种主要成分的含量
- Simultaneous Determination of Eight Major Compounds in Mailuoning Injection by HPLC
- 中国实验方剂学杂志 2012年18卷第22期页码：101-105
- 作者机构：
- 作者简介：
- 基金信息：
  
  江苏省中医药局科学技术研究专项基金(LZ11035);江苏省中医药领军人才(LJ200906);中药新药临床评价研究技术平台(南京)建设项目(2012ZX09303009-002)
- DOI：
  中图分类号：
- 纸质出版日期：2012
- 稿件说明：
移动端阅览
吴婷, 张军, 周玲, 等. HPLC同时测定脉络宁注射液中8种主要成分的含量[J]. 中国实验方剂学杂志, 2012,18(22):101-105.

WU Ting, ZHANG Jun, ZHOU Ling, et al. Simultaneous Determination of Eight Major Compounds in Mailuoning Injection by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(22): 101-105.
吴婷, 张军, 周玲, 等. HPLC同时测定脉络宁注射液中8种主要成分的含量[J]. 中国实验方剂学杂志, 2012,18(22):101-105. DOI：

WU Ting, ZHANG Jun, ZHOU Ling, et al. Simultaneous Determination of Eight Major Compounds in Mailuoning Injection by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(22): 101-105. DOI：

摘要

目的: 建立用高效液相色谱法梯度洗脱同时测定脉络宁注射液中绿原酸、新绿原酸、隐绿原酸、咖啡酸、肉桂酸、3

4-二-O-咖啡酰基奎宁酸

5-二-O-咖啡酰基奎宁酸

5-二-O-咖啡酰基奎宁酸含量的方法

并对3个批次脉络宁注射液中上述8种化合物进行定量分析。方法: 流动相乙腈-0.8%醋酸

梯度洗脱

流速1 mL·min-1

柱温30 ℃

检测波长λ1=327 nm(绿原酸、新绿原酸、隐绿原酸、咖啡酸、3

4-二-O-咖啡酰基奎宁酸

5-二-O-咖啡酰基奎宁酸

5-二-O-咖啡酰基奎宁酸)

λ2=278 nm(肉桂酸);进样量为10 μL。结果: 绿原酸、新绿原酸、隐绿原酸、咖啡酸、肉桂酸、3

4-二-O-咖啡酰基奎宁酸

5-二-O-咖啡酰基奎宁酸

5-二-O-咖啡酰基奎宁酸的线性范围分别为2.57~25.7(r=0.999 7)

2.05~20.5(r=0.999 7)

2.26~22.6(r=0.999 9)

1.92~19.2(r=0.999 9)

1.16~11.6(r=0.999 7)

2.282~22.82(r=0.999 8)

1.839~18.39(r=0.999 8)

2.194~21.94(r=0.999 7)mg·L-1

加样回收率分别为95.26%(RSD 1.21%)

96.03%(RSD 1.09%)

97.25%(RSD 2.18%)

100.98%(RSD 1.98%)

97.44%(RSD 2.38%)

97.78%(RSD 2.24%)

98.37%(RSD 2.15%)

95.82%(RSD 2.30%)。结论: 方法简便、快捷

准确、重复性好

可为脉络宁注射液提供质量控制依据。

Abstract

Objective:To establish an HPLC method for determination of eight major compounds in Mailuoning injection

including chlorogenic acid

neochlorogenic acid

cryptochlorogenic acid

caffeic acid

cinnamic acid

4-dicaffeoylquinic acid

5-dicaffeoylquinic acid and 4

5-dicaffeoylquinic acid. Method: The mobile phase was composed of acetonitrile-0.8% acetic acid with gradient elution. The flow rate was 1.0 mL·min-1. The column temperature was set at 30 ℃. The detection wavelength was set at 327 nm for chlorogenic acid

neochlorogenic acid

cryptochlorogenic acid

caffeic acid

4-dicaffeoylquinic acid

5-dicaffeoylquinic acid and 4

5-dicaffeoylquinic acid and 278 nm for cinnamic acid. The injection volume was 10 μL. Result: The linear ranges of eight compounds were as follows: 2.57-25.7 mg·L-1(r=0.999 7) for chlorogenic acid

2.05-20.5 mg·L-1(r=0.999 7) for neochlorogenic acid

2.26-22.6 mg·L-1(r=0.999 9) for cryptochlorogenic acid

1.92-19.2 mg·L-1(r=0.999 9) for caffeic acid

1.16-11.6 mg·L-1(r=0.999 7) for cinnamic acid

2.282-22.82 mg·L-1(r=0.999 8) for 3

4-dicaffeoylquinic acid

1.839-18.39 mg·L-1(r=0.999 8) for 3

5-dicaffeoylquinic acid and 2.194-21.94 mg·L-1(r=0.999 7) for 4

5-dicaffeoylquinic acid. The average recoveries were 95.26%(RSD 1.21%)

96.03%(RSD 1.09%)

97.25%(RSD 2.18%)

100.98%(RSD 1.98%)

97.44%(RSD 2.38%)

97.78%(RSD 2.24%)

98.37%(RSD 2.15%)

95.82%(RSD 2.30%)

respectively. Conclusion: The method is convenient

rapid and accurate with good reproducibility and could be used for the quality control of Mailuoning injection.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

HPLC同时测定清肝利胆口服液中7种成分

HPLC测定石韦配方颗粒中绿原酸、咖啡酸及芒果苷的含量

不同产地野菊花中3种有机酸的含量测定

HPLC测定大血藤药材中绿原酸的含量

HPLC同时测定消癌平注射液中7种主要成分的含量