Objective:To explore possible molecular mechanism of Tangluoning(TLN) formula in improving oxidative stress. Method: The SD rats were divided into control group

the others were given intraperitoneal injection of streptozocin(STZ) (60 mg·kg-1) after 12 hours of fasting to build diabetic model. The rats with blood glucose level being more than 16.7 mmol·L-1 after stop 8 hours of fasting were regarded as modelling successfully after 3 days.The rats modeling successfully were randomly divided into the model group

TLN group and alpha-lipoic acid group (LA)

15 in each group. Drugs were administrated orally for 8 weeks after the STZ treatment.Blood glucose

cool or thermal response time

thermal threshold were determined during the experimental process. After eight weeks of treatment

Real-time PCR technology was employed to measure Mn superoxide dismutase(SOD) mRNA

CuZnSOD mRNA and glutathione peroxidase(GPx) mRNA expression in the sciatic nerve. Result: The expression of MnSOD mRNA

CuZnSOD mRNA and GPx mRNA was increased after STZ treatment. Compared with the model group

the cool or thermal response time was decreased

the thermal threshold was increased

the gene expression of MnSOD

CuZnSOD and GPx was increased by TLN. Conclusion: Tangluoning formula could improve hyperalgesia

dysesthesia

increase antioxidant enzymes gene expression to improve oxidative stress.