Objective:To optimize enzymatic hydrolysis conditions of proDer f 1

and investigate allergenicity of enzymatic hydrolysate. Method: With hydrolysis degree as index

optimum hydrolysis technology of proDer f 1 was investigated by orthogonal test using papain and trypsin;Allergenicity of enzymatic hydrolysate was tested by ELISA. Result: Temperature was main factor affecting hydrolysis effect of proDer f 1

optimal hydrolysis conditions of proDer f 1 digested by papain were as follows:pH 6.5

temperature 60 ℃

hydrolysis time 4 h

the amount of papain 4 000 U·g-1;Optimum hydrolysis conditions of trypsin was pH 8.0

temperature 45 ℃

hydrolysis time 4 h

the amount of enzyme 5 000 U·g-1.Compared with proDer f 1 allergen

ELISA analysis showed that allergens were reduced significantly of papain hydrolysate and trypsin hydrolysate

allergen of trypsin hydrolysate was lower than papain hydrolysate. Conclusion: Optimized enzymatic hydrolysis process of proDer f 1 protein was feasible

and after enzymatic hydrolysis of allergen

there was different degrees reduction of allergenicity

especially change of trypsin hydrolysate had the most significant.