HPLC测定利咽解毒颗粒中黄芩苷和大黄素含量

刘阿静; 齐广才; 刘珍叶; 白梓静

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HPLC测定利咽解毒颗粒中黄芩苷和大黄素含量

更新时间：2024-01-04

- HPLC测定利咽解毒颗粒中黄芩苷和大黄素含量
- Simultaneous Determination of Baicalin and Emodin in Liyan Jiedu Granules by HPLC
- 中国实验方剂学杂志 2013年19卷第4期页码：146-149
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- 纸质出版日期：2013
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刘阿静, 齐广才, 刘珍叶, 等. HPLC测定利咽解毒颗粒中黄芩苷和大黄素含量[J]. 中国实验方剂学杂志, 2013,19(4):146-149.

LIU A-jing, QI Guang-cai, LIU Zhen-ye, et al. Simultaneous Determination of Baicalin and Emodin in Liyan Jiedu Granules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(4): 146-149.
刘阿静, 齐广才, 刘珍叶, 等. HPLC测定利咽解毒颗粒中黄芩苷和大黄素含量[J]. 中国实验方剂学杂志, 2013,19(4):146-149. DOI：

LIU A-jing, QI Guang-cai, LIU Zhen-ye, et al. Simultaneous Determination of Baicalin and Emodin in Liyan Jiedu Granules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(4): 146-149. DOI：

摘要

目的: 建立同时测定利咽解毒颗粒中黄芩苷和大黄素含量的HPLC法并通过响应曲面优化法对二者的提取工艺进行优化。方法: 采用Agilent ZORBAX SB-C18 (4.6 mm×250 mm

5 μm)色谱柱

以乙腈(A)-0.2%磷酸溶液(B)为流动相梯度洗脱

流速1.0 mL·min-1

检测波长为280 nm和245 nm

柱温28℃。结果: 黄芩苷和大黄素分别在2.016~134.4

0.408 0~27.20 mg·L-1呈良好的线性关系; 平均回收率(n=6)为 98.9%和97.8%。结论: 方法简便、准确、重复性好

具有较强的实用价值。

Abstract

Objective: To develop an HPLC method for the determination of baicalin and emodin in Liyan Jiedu granules

response surface methodology (RSM) was applied to predict optimum conditions for extraction. Method: The analysis was carried out on an Agilent ZORBAX SB-C18 column (4.6 mm×250 mm

5 μm). The mobile phase consisted of acetonitrile (A) and 0.2% phosphoric acid (B) with gradient elution at a flow rate of 1.0 mL·min-1. The detection wavelength were set at 280

245 nm

respectively. The column temperature was 28℃. Result: The linear ranges of baicalin and emodin were 2.016-134.4 and 0.408 0-27.20 mg·L-1

respectively. The average recoveries (n=6) of these were 98.9% and 97.8%

respectively. Conclusion: This method is simple

accurate and repeatable

and can be used for the quality control of Liyan Jiedu granules.

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