秋鼠曲草花部分有效成分提取工艺优选

骆冲; 孙慧; 陈芳; 赖家平

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秋鼠曲草花部分有效成分提取工艺优选

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- 秋鼠曲草花部分有效成分提取工艺优选
- Optimization of Extraction Technology for Main Components from Flower of
- 中国实验方剂学杂志 2012年18卷第19期页码：53-56
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- 纸质出版日期：2012
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骆冲, 孙慧, 陈芳, 等. 秋鼠曲草花部分有效成分提取工艺优选[J]. 中国实验方剂学杂志, 2012,18(19):53-56.

LUO Chong, SUN Hui, CHEN Fang, et al. Optimization of Extraction Technology for Main Components from Flower of [J]. Chinese journal of experimental traditional medical formulae, 2012, 18(19): 53-56.
骆冲, 孙慧, 陈芳, 等. 秋鼠曲草花部分有效成分提取工艺优选[J]. 中国实验方剂学杂志, 2012,18(19):53-56. DOI：

LUO Chong, SUN Hui, CHEN Fang, et al. Optimization of Extraction Technology for Main Components from Flower of [J]. Chinese journal of experimental traditional medical formulae, 2012, 18(19): 53-56. DOI：

摘要

目的:优选秋鼠曲草花中主要成分的提取工艺。方法:以槲皮素和芹菜素含量为综合评价指标

采用HPLC测定指标成分含量

采用单因素试验考察提取方式、料液比、提取次数及乙醇体积分数;选取提取次数、提取时间、料液比及乙醇体积分数为考察因素

正交设计试验优选提取工艺。结果:最佳提取工艺为超声提取

加入30倍量60%乙醇超声提取2次

每次40min。结论:优选的提取工艺简单、稳定可行

可为秋鼠曲草花部分有效成分的定量分析提供可靠的提取方法。

Abstract

Objective:To optimize extraction process of main components from flower of Gnaphalium hypoleucum. Method:With the contents of quercetin and apigenin as comprehensive evaluation index

which were determined by HPLC.Extraction method

ratio of solid-liquid

extraction times and the concentration of ethanol were investigated by single factor test;orthogonal design test was used to optimize extraction technology with extraction time

extraction times

ratio of solid-liquid and the concentration of ethanol. Result:Optimum extraction conditions were as follows:ultrasonic extracted 2 times with 30 times the amount of 60% ethanol

40 min per time. Conclusion:This optimized process was simple

stable and feasible.It could provide reliable extraction method for quantitative analysis of main components from flower of G. hypoleucum.

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