GE Ting-ting, CHENG Jian-ming. Determination of Homoplantaginin Content in Extract by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(23): 59-61.
GE Ting-ting, CHENG Jian-ming. Determination of Homoplantaginin Content in Extract by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(23): 59-61.DOI:
目的: 建立荔枝草提取物中高车前苷含量的高效液相(HPLC)测定方法。 方法: Hedera ODS-2 色谱柱(4.6 mm×250 mm
5 μm)
流动相甲醇-0.5%冰醋酸水溶液(50∶50)
柱温30 ℃
流速1 mL·min-1
检测波长335 nm。 结果: 高车前苷在0.20~2.01 μg呈良好的线性关系
平均回收率101.70%
RSD 1.84%。 结论: 该方法简便、准确、专属性强
可以作为荔枝草提取物中高车前苷含量测定的一种有效方法。
Abstract
Objective: To establish the assay method for determining content of homoplantaginin in extract of Salvia plebeia. Method : Hedera ODS-2 column was used with methanol-0.5% acetic acid (50∶50) as mobile phase; the column temperature was maintained at 30 ℃; the flow rate was 1 mL·min-1; detection wavelength was set at 335 nm. Result: Homoplantaginin showed a good linear relationship within 0.20-2.01 μg. The average of coefficient of recovery was 101.70%
RSD was 1.84%. Conclusion: This method is simple
accurate
specific
and can be considered as an effective method for determining content of homoplantaginin in the extract of Salvia plebeia.