Objective: To optimize infiltration and cutting technology conditions for pieces of Isatis indigotica. Method: With the content of water extract

alcohol extract and (R

S)-goitrin as indexes

water consumption

infiltration time and thickness of pieces were selected as factors

infiltration and cutting technology for pieces of I. indigoticawas optimized by orthogonal test.The content of extract and (R

S)-goitrin were determined by gravimetric method and HPLC. Result: Thickness of pieces was major factor for infiltration and cutting technology of pieces of I. indigotica

followed by infiltration time and water consumption.Optimum infiltration and cutting technology was:infiltrated 20 h with 0.6 times the amount of pieces of I. indigotica

thickness of pieces 3 mm and drying at 60℃. Conclusion: Optimized infiltration and cutting technology was stable and feasible

it could provide experimental basis for processing technology standardization and quality standard of pieces of I. indigotica.