Objective: To determine indigo blue and indirubin content in Compound South Baphicacanthus Granules by HPLC. Method: Samples were separated by Angilent C18 column (4.60 mm×150 mm

5 μm)

the mobile phase consisted of methonal-2% phosphate (by gradient elution) with flow rate at 0.9 mL·min-1

UV detection wavelength was set at 289 nm

column temperature at 35℃

and sample size was 20 μL. Result: The linear range of indigo blue was 0.030 4-0.0.608 μg (r=0.999 3)

and indirubin was 0.099 3-1.986 μg (r=0.999 6). The average recovery of indigo blue was 98.1% (RSD 2.31%)

indirubin was 101.4% (RSD 2.61%). Conclusion: The method is sensitive

simple

accurate

and can be used for the quality control of Compound South Baphicacanthus Granules.