Objective: To study the effects of tanshinone ⅡA on peripheral blood of matrix metalloproteinase (MMP-2/TIMP-2) and atrial muscle tissue of L-type calcium channel a1c subunit (LTCCa1c) effect

revealing the direct molecular mechanism in rats with atrial fibrillation. Method: The rats were randomly divided into four groups: normal control group

model group

tanshinone ⅡA treatment group and verapamil intervention group. The rat model with atrial fibrillation was tail vein injected with acetylcholine a calcium chloride (Ach-CaCl2) drug; the expressions of MMP-2 and TIMP-2 in serum were examined by enzyme-linked immunosorbent assay (ELISA)method; by western blotting in detection of atrial muscle LTCCa1c protein expression. Result: Compared with the normal control group

the model group showed higher levels of MMP-2 and reduced of TIMP-2 in the peripheral blood

while LTCCa1c of atrial muscle tissue are reduced in the protein expression levels(P<0.05).Compared with the model group

treatment group of tanshinone ⅡA and verapamil intervention group were decreased the level of MMP-2

increased TIMP-2 level

improve atrial fibrillation rats in atrial muscle tissue LTCC protein expression levels reduce state (P<0.05). Conclusion: Tanshinone ⅡA can significantly interfere with the MMP-2/TIMP-2 and LTCCa1c-protein expression

the display may through the multiple target effect

improve the degree of injury of atrial fibrillation rats atrial muscle tissue remodeling process

thereby treating atrial fibrillation.