Objective:Inducing callus and adventitious root from Scrophulariae Radix;isolating endophytes;and comparing Hapagoside yield of the different parts of Scrophulariae Radix. Method: Tender leafs of Scrophulariae Radix were cut into small pieces and sterilized to inoculate on MS

N6 mediums with different plant hormones to induce callus; Liquid medium were used in adventitious root inducing and medium transformed according to growing phase during the culture process;general method was used in endophytes isolated. Hapagoside detected with UV spectrophotometry at the wave length 255 nm. Result: Moderate density callus were induced with MS+NAA 0.05 or 0.2 or 2 mg·L-1+6-BA 2 mg·L-1 medium;mass adventitious roots obtained after 30 d’s vibrate cultivation; 4 strains of endophytes produce hapagoside were isolated from Radix Scrophulariae root; hapagoside content of callus

adventitious root and fermentation liquor of the four endophyte straints in orderly are 0.411

0.099 5

0.451

0.444

0.489

0.440 g·L-1. Conclusion: Content of hapagoside in callus is 4 times than that of adventitious root;endophytes can yield almost the same amount hapagoside as callus

and have great potential in producing secondary metabolites.