Objective: To optimize extraction technology of Phellodendrom chinense and Sophora flavescens from Fushubao gel. Method: Taking yield of matrine

berberine hydrochloride and extract as indexes

single factor test was used to choose extraction method

including water boiling method

ethanol reflux method and ethanol percolation method;Orthogonal test was designed to optimize extraction technology of P. chinense and S. flavescens from Fushubao gel with extraction times

extraction time

the amount and concentration of ethanol as factors. Result: Ethanol reflux method was selected

optimum extraction technology was as follows:extracted 3 times with 8 times the amount of 50% ethanol

1.5 hours each time.Yield of total alkaloid was about 2.47%. Conclusion: This optimized process was stable

feasible and reproducible

it could provide experimental basis for extraction of active ingredients from Fushubao gel.